class iii beta tubulin tuj1  (Proteintech)

Journal: ACS Omega

Article Title: LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids

doi: 10.1021/acsomega.4c04712

Figure Lengend Snippet: Characterization of the human cerebral organoids. Nuclei are stained blue with 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) in all images. (A) Immunostaining for 5-HT 2A receptors, which colocalize with the neuronal marker MAP2; (B) TUJ1 immunostaining (green), a neuronal marker, accompanied by PAX6 immunostaining in red, which highlights neural progenitors; (C) GFAP immunostaining identifies radial glia and astrocytes. Scale bars represent 400 μm for whole organoid images and 60 μm for zoomed-in images.

Article Snippet: Subsequently, a blocking step was carried out by incubating in a solution containing 3% fetal calf serum in PBS for 1 h. Following blocking, they were subjected to overnight incubation at 4°C with the primary antibody TUJ1 (Neuromics) at a dilution of 1:2000 in the blocking solution.

Techniques: Staining, Immunostaining, Marker

Journal: ACS Omega

Article Title: LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids

doi: 10.1021/acsomega.4c04712

Figure Lengend Snippet: Neurite Outgrowth in Response to LSD: Human brain spheroids were initially plated for 24 h and then subjected to exposure to either 10 or 100 nM LSD for an additional 24 h. For this analysis, the control group included 18 spheroids, while the groups exposed to 10 and 100 nM LSD consisted of 17 and 15 spheroids, respectively. (A) The samples were analyzed using immunofluorescence staining for β-tubulin III (TUJ1; depicted in green) and DAPI staining to highlight nuclei (shown in blue). (B) Sholl analysis was employed to assess neurite outgrowth. An inset in this section provides a schematic of the Sholl analysis method. The analysis was conducted on the mean numbers of crossings for each group of five circles. The quantification was carried out over five independent experiments. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, in comparison to the control group. A scale bar indicating 1000 μm is included for reference.

Article Snippet: Subsequently, a blocking step was carried out by incubating in a solution containing 3% fetal calf serum in PBS for 1 h. Following blocking, they were subjected to overnight incubation at 4°C with the primary antibody TUJ1 (Neuromics) at a dilution of 1:2000 in the blocking solution.

Techniques: Control, Immunofluorescence, Staining, Comparison